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KMID : 0365819730130010117
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Journal of Pusan Medical College
1973 Volume.13 No. 1 p.117 ~ p.127
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Effect of Administration Time of Prednisolone on Circulating Antibody Production to Egg Albumin in Rabbits
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Abstract
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Albino adult rabbits were injected through the ear vein two times with 5.0 ml of 1% egg albumin as an antigen for successive two days. Prednisolone treatment was started at 48-hour before and after the first injection of the antigen and simultaneously and continued on 7 successive days. On the 6th, 11th, 16th, 21st, 26th, 31st, and 36th days after the first injection of the antigen, the rabbits were bled partially and sera were pooled each experimental group. Anti-egg-albumin rabbit sera were measured by the methods of precipitation, passive hemagglutination, modified hemolytic reaction and complement fixation tests. The effect of prednisolone on the production of circulating antibodies were investigated to the anti-egg-albumin rabbit sera and the following results were obtained.
1. Production of precipitin and complement fixing antibodies were markedly suppressed when prednisolone treatment were started at 48-hour before the first injection of antigen, but slightly suppressive effect on the antibody formation when prednisolone treatment were started at 48-hour
after and simultaneous injection of antigen.
2. Antibody titers measured by passive hemagglutination and modified hemolytic reactions were paralleled with those measured by precipitation and complement fixation tests in all experimental rabbit groups, but the suppressive effect of prednisolone on the production of the former antibodies were less influenced than the later antibodies.
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